Avoiding Surface Film in Drop Checker - any tips?

Discussion in 'Carbon Dioxide (CO2)' started by Ray, 20 Oct 2008.

  1. Ray

    Ray Member

    Messages:
    474
    Location:
    Switzerland
    Does anyone else get this? Apparently although surface agitation is enough to keep the surface clear, it still forms in my DC. Once it does I get a nice yellow result 24/7 which is confusing. If I re-fill the DC with air it clears but re-forms quite quickly unless I rinse it out.

    How to stop this, should I be soaking it in bleach or boiling it or something?
     
  2. LondonDragon

    LondonDragon Administrator Staff Member

    Messages:
    10,301
    Location:
    London
    Do you use tank water to create the solution?
     
  3. Ray

    Ray Member

    Messages:
    474
    Location:
    Switzerland
    No, 4DKh, but I should have been more precise above - the surface film is not on the 4DKh/reagent mix, it is in the funnel where the air pocket in the DC meets the tank water outside.
     
  4. Ed Seeley

    Ed Seeley Member

    Messages:
    3,262
    Location:
    Nottingham
    I get it forming whenever I clean a drop checker out or use a new one. I find that after I remove it a couple of times it doesn't form again. Your drop checker's going yellow as the bacterial film (and I'm pretty sure it is a bacterial film that forms) respires CO2 so increases the CO2 concentration as well as reducing the diffusion across the water's surface in the DC.
     
  5. Ray

    Ray Member

    Messages:
    474
    Location:
    Switzerland
    I think you are right, but I was wondering, should I be sterilising my DC or something to try to stop it happening - i.e.. is it because I've got biofilm build up on the glass that spreads accross the surface?
     
  6. Ed Seeley

    Ed Seeley Member

    Messages:
    3,262
    Location:
    Nottingham
    I've always found it worse when I clean the DC! Just blow off the bacterial film for a few days and it then goes away for me! If that doesn't work then try the sterilisation and see what happens - it won't do any harm.
     

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